Coding

Part:BBa_K2272004:Design

Designed by: Eric Joshua Wang   Group: iGEM17_Virginia   (2017-10-22)


pBAD P.D. TAT(twin-arginine translocation)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 171
    Illegal NgoMIV site found at 219
    Illegal NgoMIV site found at 225
    Illegal NgoMIV site found at 240
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

As most part of the nitrification circuit functions in the periplasm of the cell, the TAT signal peptide was employed to transport large folded proteins, such as HAO and cytochrome, across the membrane.


Source

The twin-arginine translocation (Tat) protein export pathway. https://www.nature.com/articles/nrmicro2814.

References